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deff fn
2 months ago
12

An SDS lysate of red blood cells was submitted to SDS polyacrylamide gel electrophoresis. The sample revealed a major band with

a molecular weight of approximately 17,000. What protein do you think is responsible for this band?
Biology
1 answer:
enyata [2.5K]2 months ago
5 0

Respuesta:

A partir del peso molecular dado - 17,000, la proteína de los glóbulos rojos es Hemoglobina A (Hb A).

Explicación:

La técnica utilizada para la separación de moléculas de proteínas según su tamaño molecular en el estudio de biología molecular, junto con un marcador de proteína de referencia para identificar el tamaño exacto de proteína desconocida, se conoce como electroforesis en gel de poliacrilamida con SDS. Durante el proceso de separación de proteínas, ADN y ARN, entre otros, el dodecil sulfato de sodio se utiliza como un compuesto orgánico sintético empleado como tensioactivo en la solución de lisis.

Con base en el peso molecular dado - 17,000, la proteína en los glóbulos rojos es Hemoglobina A (Hb A). Su función consiste en transportar oxígeno desde los órganos respiratorios hacia el resto del cuerpo y tejidos. La Hb A se presenta en forma de tetámero, es decir, contiene cuatro subunidades que consisten en dos subunidades alfa y dos beta, cada una compuesta por 141 y 146 residuos de aminoácidos, respectivamente. Cada subunidad tiene un tamaño molecular de aproximadamente 16,000 daltones y son estructuralmente similares y del mismo tamaño.

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A hypothesis is a general principle or explanation that is derived from observations. Suppose you make the following observation
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Question continued

For each statement below, indicate "True" if it can be considered a valid hypothesis based solely on these three observations, and mark "False" if it cannot be considered valid.

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4. Certain cells contain a nucleus.

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1. The onion cells I examined contain a nucleus. false - this represents an observation based on the evidence you have collected.

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3. Every cell contains a nucleus. true  - this serves as a hypothesis since you examined 3 different cell types, taking several samples of each, and discovered they all possess a nucleus

4. Certain cells contain a nucleus. false - this cannot be seen as a valid hypothesis. The current evidence implies that all cells have a nucleus

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6. If I observe 10 skin cells from my hand, each one will contain a nucleus. false - a hypothesis should be a broad principle that is supported by evidence; this statement is overly specific.

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