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deff fn
7 days ago
12

An SDS lysate of red blood cells was submitted to SDS polyacrylamide gel electrophoresis. The sample revealed a major band with

a molecular weight of approximately 17,000. What protein do you think is responsible for this band?
Biology
1 answer:
enyata [1.7K]7 days ago
5 0

Respuesta:

A partir del peso molecular dado - 17,000, la proteína de los glóbulos rojos es Hemoglobina A (Hb A).

Explicación:

La técnica utilizada para la separación de moléculas de proteínas según su tamaño molecular en el estudio de biología molecular, junto con un marcador de proteína de referencia para identificar el tamaño exacto de proteína desconocida, se conoce como electroforesis en gel de poliacrilamida con SDS. Durante el proceso de separación de proteínas, ADN y ARN, entre otros, el dodecil sulfato de sodio se utiliza como un compuesto orgánico sintético empleado como tensioactivo en la solución de lisis.

Con base en el peso molecular dado - 17,000, la proteína en los glóbulos rojos es Hemoglobina A (Hb A). Su función consiste en transportar oxígeno desde los órganos respiratorios hacia el resto del cuerpo y tejidos. La Hb A se presenta en forma de tetámero, es decir, contiene cuatro subunidades que consisten en dos subunidades alfa y dos beta, cada una compuesta por 141 y 146 residuos de aminoácidos, respectivamente. Cada subunidad tiene un tamaño molecular de aproximadamente 16,000 daltones y son estructuralmente similares y del mismo tamaño.

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