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creativ13
2 months ago
5

Ten grams of hamburger were added to 90 ml of sterile buffer.

Biology
1 answer:
-BARSIC- [2.5K]2 months ago
7 0

Answer:

5.2 × 10 ⁹ cfu

Explanation:

Applying the dilution factors

0.1 ml of the final dilution contains 52 colonies

1 ml would thus have around 520 colonies

10 ml of the final sample would contain 5200 colonies

at a 1 / 100 dilution

1 ml of the sample holds 5200 colonies

100 ml of the sample will yield 520000 colonies

1 ml from the 0.1 ml + 9.9 ml encompasses 520000 colonies

10 ml would then have 5200000

following the second stage of the dilution

0.1 ml of the slurry contained 5200000 colonies

1 ml would yield 52000000 colonies

10 ml would lead to 520000000 colonies

100 ml of the starting sample ( 10 grams + 90 ml ) results in 5200000000 colonies =

5.2 × 10 ⁹ cfu

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