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Mariana
8 days ago
8

One genetic engineering application that relies on transgenic organisms is the production of large quantities of a desired prote

in, such as the human insulin protein. This process involves the use of restriction enzymes that act like molecular scissors to cut out the gene of interest. Another enzyme called ligase "glues" the gene into the target DNA.
Write the steps of gene cloning into the correct sequence.

1. Ligase pastes the insulin gene into the bacterial plasmid.
2. Cut out the insulin gene using restriction enzymes. Cut the bacterial plasmid with the same restriction enzymes.
3. The bacterium multiplies, producing many bacterial cells that make the insulin protein.
4. Human insulin is isolated from the transgenic bacteria.
5. The recombinant plasmid containing the Insulin gene is taken up by a bacterium.
Biology
1 answer:
11111nata11111 [2.5K]8 days ago
6 0

Answer:2,1,3,5,4

Explanation:

Transgenes refer to genes that are artificially introduced into an organism.

Transgenic organisms are those that contain transgenes. These terms are utilized in genetic engineering. The process of gene cloning or the introduction of transgenes enables individual transformations, and this technique is employed to create Genetically Modified Organisms (GMOs).

The following steps are involved:

1. Use restriction enzymes to excise the insulin gene. Additionally, cut the bacterial plasmid with the same restriction enzymes, which are considered endonucleases.

2. Ligase enzyme connects the insulin gene to the bacterial plasmid.

3. The bacterium proliferates, leading to the production of numerous bacterial cells that generate the insulin protein.

4. A bacterium absorbs the recombinant plasmid containing the insulin gene.

5. Human insulin is extracted from the transgenic bacteria.

It's important to note that for gene cloning to occur, there must be a gene to be introduced, a vector, a host cell for multiplication of that gene, a method to transform the organism, and a means to achieve a whole organism.

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