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patriot
22 days ago
8

PCR (polymerase chain reaction) is an excellent method of generating copies of target DNA. If a single piece of double stranded

DNA (dsDNA) is put into a PCR machine, how many dsDNA segments will there be after 3 rounds?
Biology
1 answer:
Tresset [1.6K]22 days ago
8 0

Answer: After completing three rounds, there will be 8 segments with 2 original strands on different segments.

Explanation:

The polymerase chain reaction (PCR) processes the double-stranded DNA (dsDNA) in a PCR machine in a specific, repeated manner.

1st round:

A single unit of double-stranded DNA (dsDNA) separates into two strands, allowing complementary nucleotides to be synthesized to create a double helix.

2nd round:

The two strands of dsDNA produce 4 segments prior to the synthesis of complementary nucleotides.

3rd round:

The four dsDNA strands lead to the creation of 8 segments before synthesis again happens.

Thus, at the conclusion of three rounds, the two original strands will appear across 8 different segments of replicated DNA.

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Choice C is correct. The folding of the inner mitochondrial membrane is essential to enhance surface area for numerous protein complexes involved in the Krebs cycle during cellular respiration. One significant protein is ATP synthase, which uses potential energy harnessed during proton transport to generate ATP. A flatter inner membrane would reduce ATP synthase quantity, compromising mitochondrial efficiency and leading to fatigue during high energy demands.
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The higher up on a cladogram animals are
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The higher an animal is positioned on a cladogram, the more closely it is related to other animals. 
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Like blood lymph flows both to and from the heart. <br> a. True <br> b. False
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8 days ago
The rate of demand for energy in the body determines whether the body will use aerobic or
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Explanation:

Isabella: 50-meter freestyle - limited oxygen supply over a brief duration

  • anaerobic cellular metabolism
  • partial breakdown of glucose results in: lactic acid
  • yields 2 ATP molecules per gram of glucose

Tyler: half marathon- sufficient oxygen supply to tissues over an extended period

  • aerobic cellular metabolism
  • full glucose breakdown
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  • waste products: H2O, CO2

Further Explanation:

in summary: C6H12O6 (glucose) + 6 O2 → 6 CO2 + 6 H2O + ≈38 ATP

Mitochondria, which are small membrane-bound organelles in all eukaryotic cells, generate most of the chemical energy necessary for cellular biochemical processes. This energy is stored as ATP, which is synthesized in the mitochondria. ATP production through respiration in the mitochondria involves oxygen in the Krebs’ or Citric acid cycle, utilizing pyruvate oxidation (which occurs through glycolysis in the cytoplasm).

Oxidative phosphorylation refers to a mechanism where the NADH and FADH2 produced earlier in the respiration process donate electrons to the electron transport chain, reverting to their original forms, NADH+ and FAD. The flow of electrons through the chain releases energy that is utilized to pump protons out of the mitochondrial matrix.

This creates a gradient with a varying number of protons on each side of the membrane, allowing protons to flow back into the matrix via the ATP synthase enzyme, converting ADP into ATP. At the electron transport chain's conclusion, three oxygen molecules accept electrons and protons, culminating in water formation...

  • Glycolysis happens in the cytoplasm, using 2 ATP to split glucose into 2 pyruvates, yielding 4 ATP and 2 NADH molecules (resulting in a net ATP gain of 2)
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In specific cellular conditions, aerobic respiration may be impeded due to various reasons:

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  • - lack of necessary genes for enzymes in the Kreb's cycle

Consequently, cells resort to alternative methods for ATP energy production and to regenerate NAD+, an oxidized form of NADH that serves as the primary electron carrier during glycolysis. Pyruvate, generated in the cytoplasm through glycolysis, also acts as an electron acceptor in the fermentation process.

Learn more about cellular life at

Learn more about cellular respiration at

8 0
1 month ago
you are a molecule of carbon. choose a starting point in the carbon cycle and describe the process you would go through to move
lana [1794]
Answer:

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3) carbon is incorporated into decomposed organisms
4) next, it enters deceased organisms and waste buried in the ground
5) millions of years later, it becomes fossilized
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if you want the entire cycle then..
7) utilized again by a tree
8) released as organic carbon (some)
9) a tree leaf is consumed by an animal, which then expels carbon either by respiration or after it dies
7 0
13 days ago
Read 2 more answers
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