Je ne peux pas vraiment expliquer la réponse à cause de la confusion présente dans votre question, mais je vous encourage à formuler des questions plus claires la prochaine fois.
Rat anti-goat coupled with Cy3. Indirect immunofluorescence microscopy is a method utilized to identify endogenous marker proteins and this method boasts high sensitivity. Secondary antibodies are obtained by immunizing the target species with antibodies from a second species. In a typical immunofluorescence protocol, secondary antibodies are conjugated with fluorophores and allowed to incubate with the primary antibody. In this scenario, the Cy3 fluorescent probe is employed to label secondary antibodies, absorbing light at 550 nm and emitting at 570 nm.
A change in membrane potential.
