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jok3333
3 days ago
12

A researcher is interested in performing indirect immunofluorescence microscopy in human tissue using an antibody raised in goat

s that can detect a human protein called PCNT. Which of the following antibodies would better work as the secondary antibody in this experiment? Goat anti-goat linked to Cy3 Rat anti-goat linked to Cy3 Human anti-human linked to Cy3 Goat anti-human linked to Cy3
Biology
1 answer:
enyata [1.7K]3 days ago
7 0
Rat anti-goat coupled with Cy3. Indirect immunofluorescence microscopy is a method utilized to identify endogenous marker proteins and this method boasts high sensitivity. Secondary antibodies are obtained by immunizing the target species with antibodies from a second species. In a typical immunofluorescence protocol, secondary antibodies are conjugated with fluorophores and allowed to incubate with the primary antibody. In this scenario, the Cy3 fluorescent probe is employed to label secondary antibodies, absorbing light at 550 nm and emitting at 570 nm.
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Answer:

The appropriate choice is C) random

Explanation:

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Other options are incorrect; for instance, option A is wrong since wind dispersal does not exhibit a consistent distribution pattern.

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1 month ago
The replication cycle of T7 virus has several things that none of the other virus replication cycles include. What is one of the
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The lysogenic replication cycle is a distinct procedure in the replication of the T7 virus.

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