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jok3333
2 months ago
12

A researcher is interested in performing indirect immunofluorescence microscopy in human tissue using an antibody raised in goat

s that can detect a human protein called PCNT. Which of the following antibodies would better work as the secondary antibody in this experiment? Goat anti-goat linked to Cy3 Rat anti-goat linked to Cy3 Human anti-human linked to Cy3 Goat anti-human linked to Cy3
Biology
1 answer:
enyata [2.5K]2 months ago
7 0
Rat anti-goat coupled with Cy3. Indirect immunofluorescence microscopy is a method utilized to identify endogenous marker proteins and this method boasts high sensitivity. Secondary antibodies are obtained by immunizing the target species with antibodies from a second species. In a typical immunofluorescence protocol, secondary antibodies are conjugated with fluorophores and allowed to incubate with the primary antibody. In this scenario, the Cy3 fluorescent probe is employed to label secondary antibodies, absorbing light at 550 nm and emitting at 570 nm.
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