Answer:2,1,3,5,4
Explanation:
Transgenes refer to genes that are artificially introduced into an organism.
Transgenic organisms are those that contain transgenes. These terms are utilized in genetic engineering. The process of gene cloning or the introduction of transgenes enables individual transformations, and this technique is employed to create Genetically Modified Organisms (GMOs).
The following steps are involved:
1. Use restriction enzymes to excise the insulin gene. Additionally, cut the bacterial plasmid with the same restriction enzymes, which are considered endonucleases.
2. Ligase enzyme connects the insulin gene to the bacterial plasmid.
3. The bacterium proliferates, leading to the production of numerous bacterial cells that generate the insulin protein.
4. A bacterium absorbs the recombinant plasmid containing the insulin gene.
5. Human insulin is extracted from the transgenic bacteria.
It's important to note that for gene cloning to occur, there must be a gene to be introduced, a vector, a host cell for multiplication of that gene, a method to transform the organism, and a means to achieve a whole organism.
Option C is the right choice. Since the start of industrialization in the 1800s, the concentration of carbon dioxide in the atmosphere has risen <span>. This increase is attributed to emissions from industrial machinery and vehicles.
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