Response:
The cuvette was prepared with the solution so that the spectrometer measures solely the absorbance from the solute. This also means the spectrometer disregards other fluctuations in absorbance that typically arise from the composition of water. The spectrometer focused only on the absorbance of as reflected in the spectrum. The reaction between the
and the
produces two clear liquids that generate the orange product
which leads to the absorbance spectrum. Due to the orange color of the solution, it reflects this hue and others like it, while absorbing blue tones. To isolate the absorption of just the
, pre-rinsing the cuvette with the solutions intended for measurement is advisable, along with using a kimwipe to clean any fingerprints that might impact data collection.
Clarification:
The cuvette was prepared with the solution so that the spectrometer measures solely the absorbance from the solute. This also means the spectrometer disregards other fluctuations in absorbance that typically arise from the composition of water. The spectrometer focused only on the absorbance of as reflected in the spectrum.