You want to prepare a solution with a concentration of 200.0μM from a stock solution with a concentration of 500.0mM. At your di

Question

3 months ago

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You want to prepare a solution with a concentration of 200.0μM from a stock solution with a concentration of 500.0mM. At your di

sposal, you have 5-mL and 10-mL transfer pipets and volumetric flasks of sizes 100, 250, 500, and 1000mL. Which of the following serial dilutions will give you the 200.0μM solution?

Chemistry

1 answer:

lorasvet [2.7K] · Answer 1 · 2026-02-24T14:31:26+03:00

Answer:

1) This dilution plan will yield a 200μM solution.

2) This dilution plan will not yield a 200μM solution.

3) This dilution plan will not yield a 200μM solution.

4) This dilution plan will yield a 200μM solution.

5) This dilution plan will yield a 200μM solution.

Explanation:

Convert the initial molarity into molar form as shown.

$500mM = 500mM \times (\frac{1M}{1000M})= 0.5M$

Let's examine the following serial dilution processes.

1)

Dilute 5.00 mL of the stock solution to 500 mL. Then take 10.00 mL of this new solution and dilute it further to 250 mL.

Concentration of 500 mL solution:

$M_{2}= \frac{M_{1}V_{1}}{V_{2}}= \frac{(0.5M)(5.00mL)}{500 mL}= 5 \times 10^{-3}M$

10 mL of this solution is further diluted to 250 mL

$M_{final}= \frac{M_{2}V_{2}}{V_{final}}= \frac{(5 \times 10^{-3}M)(10.0mL)}{250 mL}= 2 \times 10^{-4}M$

Convert μM:

$2 \times 10^{-4}M = (2 \times 10^{-4}M)(\frac{1 \mu M}{10^{-6}M})= 200 \mu M$

Thus, this dilution scheme will yield a 200μM solution.

2)

Dilute 5.00 mL of the stock solution to 100 mL. Then take 10.00 mL of this new solution and dilute to 1000 mL.

Concentration of 100 mL solution:

$M_{2}= \frac{M_{1}V_{1}}{V_{2}}= \frac{(0.5M)(5.00mL)}{100 mL}= 2.5 \times 10^{-2}M$

10 mL of this solution is further diluted to 1000 mL

$M_{final}= \frac{M_{2}V_{2}}{V_{final}}= \frac{(2.5 \times 10^{-2}M)(10.0mL)}{1000 mL}= 2.5 \times 10^{-4}M$

Convert μM:

$2.5 \times 10^{-4}M = (2.5 \times 10^{-4}M)(\frac{1 \mu M}{10^{-6}M})= 250 \mu M$

Thus, this dilution scheme will not yield a 200μM solution.

3)

Dilute 10.00 mL of the stock solution to 100 mL, followed by diluting 5 mL of that new solution to 100 mL.

Concentration of 100 mL solution:

$M_{2}= \frac{M_{1}V_{1}}{V_{2}}= \frac{(0.5M)(10mL)}{100 mL}= 0.05M$

5 mL of this solution is diluted to 1000 mL

$M_{final}= \frac{M_{2}V_{2}}{V_{final}}= \frac{(0.05M)(5mL)}{1000 mL}= 0.25 \times 10^{-4}M$

Convert μM:

$0.25 \times 10^{-4}M = (0.25 \times 10^{-4}M)(\frac{1 \mu M}{10^{-6}M})= 25 \mu M$

Thus, this dilution scheme will not yield a 200μM solution.

4)

Dilute 5 mL of the stock solution to 250 mL. Then take 10 mL of this new solution and further dilute it to 500 mL.

Concentration of 250 mL solution:

$M_{2}= \frac{M_{1}V_{1}}{V_{2}}= \frac{(0.5M)(5mL)}{250 mL}= 0.01M$

10 mL of this solution is further diluted to 500 mL

$M_{final}= \frac{M_{2}V_{2}}{V_{final}}= \frac{(0.01M)(10mL)}{500 mL}= 2 \times 10^{-4}M$

Convert μM:

$2 \times 10^{-4}M = (2 \times 10^{-4}M)(\frac{1 \mu M}{10^{-6}M})= 200 \mu M$

Thus, this dilution scheme will yield a 200μM solution.

5)

Dilute 10 mL of the stock solution to 250 mL. Then take another 10 mL of this new solution and dilute it to 1000 mL.

Concentration of 250 mL solution:

$M_{2}= \frac{M_{1}V_{1}}{V_{2}}= \frac{(0.5M)(10mL)}{250 mL}= 0.02M$

10 mL of this solution is further diluted to 1000 mL

$M_{final}= \frac{M_{2}V_{2}}{V_{final}}= \frac{(0.02M)(10mL)}{1000 mL}= 2 \times 10^{-4}M$

Convert μM:

$2 \times 10^{-4}M = (2 \times 10^{-4}M)(\frac{1 \mu M}{10^{-6}M})= 200 \mu M$

Thus, this dilution scheme will yield a 200μM solution.