answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Natalija
3 months ago
5

For each set of dilutions (bleach and mouthwash), the first tube contained a 1:11 dilution (0.5 mL of agent was added to 5.0 mL

of nutrient broth). What was the dilution of the MIC for the mouthwash and the bleach? Hint: You will have to calculate the dilution of each tube 2 – 4.
Chemistry
1 answer:
eduard [2.7K]3 months ago
5 0

Response:

Tube 2: 8.26 * 10^-3; Tube 4: 6.83 * 10^-5

Explanation:

For the MIC test's serial dilutions, each tube should contain an equal volume of nutrient broth: 5.0 mL, while the agent's volume per dilution must also match: 0.5 mL.

The serial dilution process followed was:

  • Tube 1: 0.5/5.5
  • Tube 2: 0.5 mL from tube 1 was diluted with 5.0 mL of broth, resulting in a dilution of tube 2 as (1:11) * (1:11) = (0.5/5.5) * (0.5/5.5) = 1:121 = 8.26 * 10^-3
  • Tube 3: similar calculations yield 1:1331 = 7.51 * 10^-4
  • Tube 4: yields 1:14641 = 6.83 * 10^-5.

You might be interested in
The density of air under ordinary conditions at 25°C is 1.19 g/L. How many kilograms of air are in a room that measures 10.0 ft
alisha [2963]

Answer: The air in the room weighs 37.068 kg

Explanation:

Given dimensions:

Length = 10.0 ft

Width = 11.0 ft

Height = 10.0 ft

The volume of the room (rectangular prism) is calculated using:

V=lbh

where l = length, b = breadth, h = height.

Substituting the values,

V=10.0ft\times 11.0ft\times 10.0ft=1100ft^3=31148.53L

Using the conversion: 1ft^3=28.3168L

Next, calculate the mass of the air based on density:

Density=\frac{Mass}{Volume}

1.19g/L=\frac{Mass}{31148.53L}

Mass=37066.7507g=37.068kg

Conversion: 1 kg = 1000 g

Thus, the air mass in the room equals 37.068 kilograms.

3 0
4 months ago
Read 2 more answers
Peter performed an experiment to test which of the three compounds his teacher gave him was an ionic compound. The table shows h
lions [2927]
B is identified as an ionic compound. Explanation: This conclusion is drawn based on the characteristics of ionic compounds, which typically form crystals similar to salt (sodium chloride), are soluble in water, exhibit high boiling and melting points, possess hardness from strong intermolecular forces, and can conduct electricity—though only when dissolved or in a molten state.
5 0
2 months ago
Read 2 more answers
Help on part "c": The forensic technician at a crime scene has just prepared a luminol stock solution by adding 19.0g of luminol
lions [2927]

1. The luminol stock solution has a molarity of 1.431 M.

2. In 2.00 L of the diluted spray, there are 0.12 moles of luminol.

3. The volume of the stock solution from Part A that contains the same number of moles present in the diluted solution from Part B is 83.86 ml.

Additional Information

Stoichiometry in Chemistry focuses on the quantitative aspects of chemical reactions, which includes calculations related to volume, mass, and the count of ions, molecules, and elements.

Key concepts in stoichiometry include:

  • 1. Relative atomic mass
  • 2. Relative molecular mass

This refers to the relative atomic mass of a molecule.

  • 3. Mole

A mole represents the number of particles in a substance equivalent to the number of atoms in 12 grams of carbon-12.

1 mole = 6.02 × 10²³ particles.

The quantity of moles can also be derived by dividing mass (in grams) by either the relative mass of an element or the relative mass of a molecule.

\large{\boxed{\bold{mol\:=\:\frac{grams}{ relative\:mass} }}}

Luminol (C₈H₇N₃O₂) is utilized for detecting blood traces at crime scenes, due to its reaction with iron found in blood.

To prepare a luminol stock solution, 19.0 g of luminol is mixed into a total volume of 75.0 mL of water.

Thus, the molarity is calculated as:

  • 1. Moles of Luminol

- the relative molecular mass of Luminol:

= 8.C + 7.H + 3.N + 2.16

= 8.12 + 7.1 + 3.14 + 2.16

= 177 grams/mol.

Thus, we have:

moles = grams / relative molecular mass.

mole=\frac{19}{177}

moles = 0.1073.

2. Molarity (M)

M = moles / volume

M\:=\:{\frac{ 0.1703 }{75.10^{-3} L}

M = 1.431.

  • b. The concentration of luminol in the spray bottle is 6.00 × 10⁻² M. Therefore, in a 2 L solution, the number of moles is:

moles = M × volume

moles = 6 × 10⁻² × 2

moles = 0.12.

  • c. The molarity of the stock solution (Part A) is 1.431 M.

The diluted solution (Part B) contains 0.12 moles of luminol.

To find the volume of the stock solution (Part A) that has the same moles as the diluted solution (Part B):

volume = moles / M

volume\:=\:\frac{0.12}{1.431}

volume = 0.08386 L = 83.86 mL.

Further Learning

moles of water you can generate

the amount of each atom in the chemical's formula

the proportion of hydrogen to oxygen atoms in 2 L of water

Keywords: mole, volume, molarity, Luminol, relative molecular mass

6 0
3 months ago
Read 2 more answers
Other questions:
  • Leonard adds 5 grams of sugar to 1 liter of water to make one sugar solution. Then he adds 10 grams of sugar to 1 liter of water
    9·1 answer
  • According to the VSEPR model, the arrangement of electron pairs around NH3 and CH4 is A. the same, because in each case there ar
    8·1 answer
  • What functional feature(s) does the phosphate group contribute to the structure of a phospholipid? select all that apply. select
    13·1 answer
  • The solid compound, K2SO4, contains?
    5·1 answer
  • A) A cat dish has a mass of 1975 g and a volume of 7500ml. What is the overall density of the cat dish? B) find the maximum mass
    6·1 answer
  • Object A has a temperature of 287 Kelvin, object B has a temperature of 374 Kelvin. In what direction will thermal energy flow?
    12·2 answers
  • 131i has a half-life of 8.04 days. assuming you start with a 1.53 mg sample of 131i, how many mg will remain after 13.0 days ___
    6·1 answer
  • A mass of chemicals contains 100 units of potential energy. after an explosion, all the chemical energy is converted into therma
    6·1 answer
  • The vapor pressure of diethyl ether (ether) is 463.57 mm Hg at 25 °C. A nonvolatile, nonelectrolyte that dissolves in diethyl et
    10·1 answer
  • Match the theoretical motions of particles with their associated phases. Solid, Gas, Liquid. 1. oscillation about a fixed point
    7·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!